LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1814..1910) /label=attL2 /note="attL2 (c)" misc_signal complement(1925..1944) /note="T7 Promoter/priming site (c)" primer_bind 1952..1968 /note="M13 Reverse priming site" gene 2081..2890 /note="Kanamycin resistance gene" rep_origin 3011..3684 /note="pUC origin" PCR_frame 669..1814 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1834) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1831) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1822..3687,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1792..1812 /note="TEV site" source 673..1791 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:21948 IMAGE:4390876" /tissue_type="Bone, osteosarcoma" /clone_lib="NIH_MGC_86" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..1791 /gene="GTDC1" /gene_synonym=mat-Xa /db_xref="GeneID:79712" /db_xref="HGNC:20887" /db_xref="MIM:610165" CDS 673..1791 /dnas_title="glycosyltransferase-like domain containing 1" /gene="GTDC1" /gene_synonym=mat-Xa /codon_start=1 /product="glycosyltransferase-like domain containing 1" /protein_id="AAH17741.1" /db_xref="GI:34190921" /db_xref="GeneID:79712" /db_xref="HGNC:20887" /db_xref="MIM:610165" /translation="MSILIIEAFYGGSHKQLVDLLQEELGDCVVYTLPAKKWHWRART SALYFSQTIPISEHYRTLFASSVLNLTELAALRPDLGKLKKILYFHENQLIYPVKKCQ ERDFQYGYNQILSCLVADVVVFNSVFNMESFLTSMGKFMKLIPDHRPKDLESIIRPKC QVIYFPIRFPDVSRFMPKHKTTHLKKMLGLKGNGGAVLSMALPFQPEQRDSEDLLKNF NSECDTHCGLDTARQEYLGNSLRQESDLKKSTSSDNSSSHHGENKQNLTVDPCDILGG VDNQQRLLHIVWPHRWLEAVYCGCYPLCPKDLVYPEIFPAEYLYSTPEQLSKRLQNFC KRPDIIRKHLYKGEIAPFSWAALHGKFRSLLTTEPREDL" misc_feature 673..1791 /note="GTDC1 (isoform 2) coding region " ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgagtat cctcatcatt gaagcattct atggaggctc ccataaacag 721 ctggtggatc ttcttcaaga agagttagga gactgtgtcg tttataccct tcctgcaaag 781 aaatggcatt ggagagcccg gacatctgct ttatatttct ctcagaccat tcccatcagt 841 gagcattaca ggaccctctt tgcaagttca gtgcttaacc tgaccgaact ggctgccctt 901 cggcctgacc ttgggaaact gaaaaagatt ctgtattttc acgagaacca gttgatatat 961 cctgtcaaga aatgtcagga gagggatttc caatatggat acaaccaaat tctttcatgc 1021 ctggtggctg atgtggttgt attcaactca gtttttaata tggaatcatt tctcacttcc 1081 atgggaaaat ttatgaagct gattcctgat cacagaccca aggatctgga aagcatcatc 1141 agacccaagt gccaagttat ttactttccc atcaggtttc ctgatgtgag cagattcatg 1201 cccaagcaca aaacaaccca tttaaagaag atgctcggcc ttaaaggaaa tggcggtgcg 1261 gttctgtcca tggcccttcc ttttcagcca gagcagagag attcagagga tttattgaag 1321 aattttaatt cagagtgtga tacacactgt ggccttgata ctgcacgaca agaatatttg 1381 ggtaactcat taagacagga atcagacttg aaaaaatcca cctcgtcaga taattcaagc 1441 tctcatcatg gtgaaaataa acaaaatctg actgttgatc cctgtgacat tttgggtgga 1501 gttgataatc agcaaagact gctacacatt gtctggcctc acaggtggtt ggaagctgtg 1561 tactgtgggt gttacccact ttgtcctaaa gatttggttt atcccgaaat atttccagct 1621 gaatatctgt attctacacc tgaacagctt tcaaaaaggc tccagaattt ctgcaagaga 1681 ccagatatta taagaaaaca tctctataag ggtgaaatcg ctccgttttc ttgggcagcc 1741 ctacatggta aattcaggtc tctgcttaca acagaaccta gggaagattt ggaaaacttg 1801 tactttcaag gctCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 1861 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC 1921 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC 1981 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT 2041 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA 2101 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC 2161 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC 2221 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT 2281 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG 2341 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT 2401 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG 2461 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT 2521 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA 2581 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC 2641 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG 2701 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT 2761 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA 2821 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA 2881 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG 2941 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG 3001 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 3061 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3121 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3181 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3241 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 3301 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 3361 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 3421 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 3481 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 3541 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 3601 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 3661 CTTTTGCTGG CCTTTTGCTC ACATGTT //